Antihyperglycemic Activity of Ethanol Extract of Robusta Coffee Beans (Coffea canephora) in Mice Induced by Streptozotocin-Nicotinamide

Abstract

Diabetes mellitus is a disease that occurs due to damage to pancreatic beta cells, which cells that are responsible for producing the hormone insulin greatly affects the metabolism of glucose in the body. Coffee robusta beans are known to have chlorogenic acid, which is a derivative of a class of flavonoid compounds which have antioxidant activity that can be used as an antihyperglycemic. to find out whether robusta coffee beans have antihyperglycemic activity. The study was conducted using the antihyperglycemic method in mice induced by streptozotocin-nicotinamide. The streptozotocy-nicotinamide-induced mice were divided into 4 categories consisting of 3 male mice each, group 1 (negative control) was only given CMC 1%, category 2 (positive control) was given glibenclamide 0.65 mg/kg, category 3 was given an ethanol extract of coffee robusta beans 200 mg/kg, category 4 they were given ethanol extract of coffee robusta beans 400 mg/kg. Blood glucose was measured on days 7, 14 and 21 after induction. The results obtained from the Robusta coffee bean extract at a dose of 200mg/kg and 400mg/kg  has activity in reducing blood glucose levels in male mice that have been induced by STZ-Na.